PhD in Biotechnology Engineering
Ben-Gurion University of the Negev
Dissertation Topic: Design of Cell Microenvironment for Controlled Osteocchondral
Differentiation of Human Mesenchymal Stem Cells.
Tali Re'em completed her PhD studies at Ben-Gurion University of the Negev in the field of Biotechnology Engineering. Her research focused on stem cells and their utilization for cartilage and bone tissue engineering. She investigated the potential use of artificial microenvironments, designed by implementing some bio-inspired components of the natural stem cell niche, for controlling the differentiation of human mesenchymal stem cell (hMSCs), and their eventual application to repair cartilage and osteochondral defects. Various elements of the native ECM were incorporated into alginate matrix, including the cell adhesive peptides and affinity-binding sites for the spatio-temporal presentation of growth factors. The research results demonstrate how the delicate design of bioactive biomaterials enables efficient hMSC differentiation, and also how these materials have a great potential as a-cellular treatment for the induction of endogenous regeneration, facilitated by host's stem cell stimulation. The presented results have major relevance for repairing cartilage and osteochondral defects in human. The research and scientific merits are summarized in a book chapter and two articles. In addition, scientific work was presented at a number of national and international meetings.
These days Tali works as a lecturer at Azrieli - College of Engineering, Jerusalem, Department of Pharmaceutical Engineering.
A Personal Perspective:
In Tali's words: "...I had the honor of being an Azrieli Fellow in the years 2008-2011. Azrieli was much more than a financial scholarship. Being a part of this program, I had the chance to meet wonderful young scientists who I believe will be the ones to lead our future top research here and abroad. I loved very much the intimate meetings, the exposure to so many research topics and fields. As an Azrieli Fellow, the financial aid and most importantly the warm and supporting atmosphere made it possible for me to really focus on my research and to get the best out of it. I was also encouraged to volunteer and enjoyed giving back to the community".
• Re'em T, Witte F, Ruvinov E, Cohen S. 2012. Simultaneous regeneration of articular cartilage and subchondral bone induced by spatially-presented TGF-beta and BMP-4 in a bi-layer affinity binding system. Acta Biomaterialia, 8 (9): 3283-93.
• Re'em T, Kaminer-Israeli Y, Ruvinov E, Cohen S. 2012. Chondrogenesis of hMSC in affinity-bound TGF-beta scaffolds. Biomaterials, 33: 751-761
• Levy O, Ruvinov E, Re’em T, Granot Y. Cohen, S. 2010. Highly efficient osteogenic differentiation of human mesenchymal stem cells by eradication of STAT3 signaling. The International Journal of Biochemistry & Cell Biology. 42 (11): 1823-30
• Re'em T, Tsur-Gang O, Cohen S. 2010. The effect of immobilized RGD peptide in macroporous alginate scaffolds on TGFβ1-induced chondrogenesis of human mesenchymal stem cells. Biomaterials, 31 (26): 6746-6755
Chapters in Books:
• Re'em T and Cohen S. Microenvironment design for stem cell fate determination. Tissue Engineering III: Cell - Surface Interactions for Tissue Culture. In Cornelia Kasper, Frank Witte, Ralf Pörtner Editors, Advances in Biochemical Engineering / Biotechnology . Springer Publishing Company. 2012. 126/2012: 227-262. DOI: 10.1007/10_2011_1180 (ISBN: 978-3-642-28281-2)
I had the opportunity to meet young high-school students from Beer Sheva and the area, and to be their guide for one day in the genetic engineering laboratory in Biotechnology Department of Ben-Gurion University. By exposing them to university research, we aimed to encourage their curiosity and ambition, and inspire them towards academic studies later on.